Bacterial Rna Thermometers Molecular Zippers And Switches Pdf


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11.04.2021 at 18:03
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bacterial rna thermometers molecular zippers and switches pdf

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RNA thermometers RNATs are cis-encoded regulatory elements that modulate translational efficiently in response to environmental temperature.

Expression of several heat shock operons, mainly coding for small heat shock proteins, is under the control of ROSE repression of heat shock gene expression in various rhizobial species. This negatively cis-acting element confers temperature control by preventing expression at physiological temperatures. We provide evidence that ROSE-mediated regulation occurs at the post-transcriptional level.

A mRNA-based thermosensor controls expression of rhizobial heat shock genes

Temperature is an important parameter that free-living cells monitor constantly. The expression of heat-shock, cold-shock and some virulence genes is coordinated in response to temperature changes. Apart from protein-mediated transcriptional control mechanisms, translational control by RNA thermometers is a widely used regulatory strategy. RNA thermometers are complex RNA structures that change their conformation in response to temperature. Melting of the structure at increasing temperature permits ribosome access and translation initiation. Different cis -acting RNA thermometers and a trans -acting thermometer will be presented. Free-living microorganisms are frequently exposed to changing environmental conditions.

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Temperature-Dependent Regulation of Bacterial Gene Expression by RNA Thermometers

Frequent transitions of bacterial pathogens between their warm-blooded host and external reservoirs are accompanied by abrupt temperature shifts. Enteric Yersiniae are Gram-negative pathogens accountable for self-limiting gastrointestinal infections. Various translational gene fusions demonstrated that this region faithfully regulates translation initiation regardless of the transcription start site, promoter or reporter strain. Consistent with translational control in bacteria, toeprinting primer extension inhibition experiments in vitro showed increased ribosome binding at elevated temperature. To assess the in vivo relevance of temperature control, we used a mouse infection model.

Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. Some RNATs act like zippers that open and close, in a reversible manner, according to the ambient temperature. These RNATs control heat shock and virulence genes.


PDF | Bacteria use complex strategies to coordinate temperature-dependent gene expression. Many genes encoding heat shock proteins and.


RNA thermometers.

Either your web browser doesn't support Javascript or it is currently turned off. In the latter case, please turn on Javascript support in your web browser and reload this page. Read article at publisher's site DOI : Ahmed R , Duncan RF. J Biol Chem, 48

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Frequent transitions of bacterial pathogens between their warm-blooded host and external reservoirs are accompanied by abrupt temperature shifts. Enteric Yersiniae are Gram-negative pathogens accountable for self-limiting gastrointestinal infections. Various translational gene fusions demonstrated that this region faithfully regulates translation initiation regardless of the transcription start site, promoter or reporter strain. Consistent with translational control in bacteria, toeprinting primer extension inhibition experiments in vitro showed increased ribosome binding at elevated temperature. To assess the in vivo relevance of temperature control, we used a mouse infection model. We conclude with a model, in which the RNA thermometer acts as translational roadblock in a two-layered regulatory cascade that tightly controls provision of the CNF Y toxin during acute infection.

Metrics details. The microbial production of rhamnolipids has been in the focus of research for the last decades. Today, mainly heterologous production systems are targeted due to the advantage of non-pathogenic hosts as well as uncoupling from complex quorum sensing regulatory networks compared to their natural producer Pseudomonas aeruginosa. In this study, the temperature-induced regulation of this native RNA-thermometer for heterologous rhamnolipid production was evaluated and its potential application for process control is discussed. For this purpose, the non-pathogenic production host P. The system was evaluated and characterized regarding the effect of temperature on growth and product formation, as represented by efficiency parameters and yields. Experimental data suggests a major effect of temperature on specific rhamnolipid production rates.

Introduction: RNA sensors

Published in Nature Reviews Microbiology Kortmann , F. Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. DOI: Kortmann and F. Bacteria use complex strategies to coordinate temperature-dependent gene expression.

Bacterial RNA thermometers: molecular zippers and switches

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